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mouse anti titin  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse anti titin
    Mouse Anti Titin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+titin/Titin+Antibody/pm40057796-57-84-86
    Average 92 stars, based on 17 article reviews
    mouse anti titin - by Bioz Stars, 2026-11
    92/100 stars

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    Related Articles

    Incubation:

    Article Title: Weaving Hypothesis of Cardiomyocyte Sarcomeres
    Article Snippet: .. Sections were incubated with goat anti-N-cadherin (N-19: sc-1502; 1:500, Santa Cruz Biotechnology Inc, Santa Cruz, CA), mouse anti-myosin (M8421: skeletal, slow; 1:500, Sigma-Aldrich Co, St. Louis, MO), mouse anti-HHF35 (M0635: actin, muscle, 1:50, Dako, Tokyo, Japan), mouse anti-desmin (N-1526: ready-to-use; Dako, Tokyo, Japan), mouse anti-titin (G-H5: sc-100959; 1:50, Santa Cruz Biotechnology Inc, Santa Cruz, CA); and goat anti-nebulin (N-19: sc-8083; 1:50, Santa Cruz Biotechnology Inc, Santa Cruz, CA) antibodies for 40 minutes at room temperature, followed by incubation for 30 minutes with peroxidase-conjugated polymer reagent (Histofine simple stain; Nichirei Bioscience Inc, Tokyo, Japan). .. The staining was visualized with diaminobenzidine (Dako, Tokyo, Japan).

    Polymer:

    Article Title: Weaving Hypothesis of Cardiomyocyte Sarcomeres
    Article Snippet: .. Sections were incubated with goat anti-N-cadherin (N-19: sc-1502; 1:500, Santa Cruz Biotechnology Inc, Santa Cruz, CA), mouse anti-myosin (M8421: skeletal, slow; 1:500, Sigma-Aldrich Co, St. Louis, MO), mouse anti-HHF35 (M0635: actin, muscle, 1:50, Dako, Tokyo, Japan), mouse anti-desmin (N-1526: ready-to-use; Dako, Tokyo, Japan), mouse anti-titin (G-H5: sc-100959; 1:50, Santa Cruz Biotechnology Inc, Santa Cruz, CA); and goat anti-nebulin (N-19: sc-8083; 1:50, Santa Cruz Biotechnology Inc, Santa Cruz, CA) antibodies for 40 minutes at room temperature, followed by incubation for 30 minutes with peroxidase-conjugated polymer reagent (Histofine simple stain; Nichirei Bioscience Inc, Tokyo, Japan). .. The staining was visualized with diaminobenzidine (Dako, Tokyo, Japan).

    Staining:

    Article Title: Weaving Hypothesis of Cardiomyocyte Sarcomeres
    Article Snippet: .. Sections were incubated with goat anti-N-cadherin (N-19: sc-1502; 1:500, Santa Cruz Biotechnology Inc, Santa Cruz, CA), mouse anti-myosin (M8421: skeletal, slow; 1:500, Sigma-Aldrich Co, St. Louis, MO), mouse anti-HHF35 (M0635: actin, muscle, 1:50, Dako, Tokyo, Japan), mouse anti-desmin (N-1526: ready-to-use; Dako, Tokyo, Japan), mouse anti-titin (G-H5: sc-100959; 1:50, Santa Cruz Biotechnology Inc, Santa Cruz, CA); and goat anti-nebulin (N-19: sc-8083; 1:50, Santa Cruz Biotechnology Inc, Santa Cruz, CA) antibodies for 40 minutes at room temperature, followed by incubation for 30 minutes with peroxidase-conjugated polymer reagent (Histofine simple stain; Nichirei Bioscience Inc, Tokyo, Japan). .. The staining was visualized with diaminobenzidine (Dako, Tokyo, Japan).

    Blocking Assay:

    Article Title: Endogenous TDP-43 mislocalization in a novel knock-in mouse model reveals DNA repair impairment, inflammation, and neuronal senescence
    Article Snippet: .. After blocking with 5% skimmed milk solution in 1% Tris-Buffered saline with Tween 20 (TBST) buffer, the membranes were immunoblotted with mouse anti-Flag antibody (Sigma, #F3165, 1:1000), rabbit anti-TDP-43 (Proteintech, #10,782–2-AP, 1:1000), mouse anti-phospho-Histone H2AX (S139) (EMD Millipore, #16–193, 1:1000), rabbit anti-H2AX (Cell Signaling Tech, #2595, 1:1000), rabbit anti-phospho-ATM (S1981) (Abcam, #ab81292, 1:800), rabbit anti-ATM (Abcam, #ab32420, 1:1000), rabbit anti-phospho-53BP1 (S1778) (Cell Signaling Tech, #2675, 1:1000), rabbit anti-53BP1 (Cell Signaling Tech, #4937, 1:1000), mouse anti-TDP-43 (R&D systems, #MAB7778, 1:1000), rabbit anti-phospho-TDP-43 (S409/410) (Proteintech, #22,309–1-AP), mouse anti-Titin (Santa Cruz Biotech, #sc-271946, 1:200), rabbit anti-Stathmin 2 (STMN2; Invitrogen, #720,178, 1:500), mouse anti-GAPDH (Novus, #NBP1-47,339, 1:2000), rat anti-Tubulin (Abcam, #ab6160, 1:2000), and mouse anti-β-actin (Proteintech, #66,009–1-Ig, 1:5000) antibodies. .. Protein bands were visualized by probing with corresponding HRP-conjugated or IRDye secondary antibodies and developed with enhanced chemiluminescence reagent or at appropriate IR channels in Odyssey (LI-COR).

    Article Title: Endogenous TDP-43 mislocalization in a novel knock-in mouse model reveals DNA repair impairment, inflammation, and neuronal senescence.
    Article Snippet: .. After blocking with 5% skimmed milk solution in 1% Tris-Buffered saline with Tween 20 (TBST) buffer, the membranes were immunoblotted with mouse anti-Flag antibody (Sigma, #F3165, 1:1000), rabbit anti-TDP-43 (Proteintech, #10,782–2-AP, 1:1000), mouse anti-phospho-Histone H2AX (S139) (EMD Millipore, #16–193, 1:1000), rabbit anti-H2AX (Cell Signaling Tech, #2595, 1:1000), rabbit anti-phospho-ATM (S1981) (Abcam, #ab81292, 1:800), rabbit anti-ATM (Abcam, #ab32420, 1:1000), rabbit anti-phospho-53BP1 (S1778) (Cell Signaling Tech, #2675, 1:1000), rabbit anti-53BP1 (Cell Signaling Tech, #4937, 1:1000), mouse anti-TDP-43 (R&D systems, #MAB7778, 1:1000), rabbit anti-phosphoTDP-43 (S409/410) (Proteintech, #22,309–1-AP), mouse anti-Titin (Santa Cruz Biotech, #sc-271946, 1:200), rabbit anti-Stathmin 2 (STMN2; Invitrogen, #720,178, 1:500), mouse anti-GAPDH (Novus, #NBP1-47,339, 1:2000), rat anti-Tubulin (Abcam, #ab6160, 1:2000), and mouse antiβ-actin (Proteintech, #66,009–1-Ig, 1:5000) antibodies. .. After blocking with 5% skimmed milk solution in 1% Tris-Buffered saline with Tween 20 (TBST) buffer, the membranes were immunoblotted with mouse anti-Flag antibody (Sigma, #F3165, 1:1000), rabbit anti-TDP-43 (Proteintech, #10,782–2-AP, 1:1000), mouse anti-phospho-Histone H2AX (S139) (EMD Millipore, #16–193, 1:1000), rabbit anti-H2AX (Cell Signaling Tech, #2595, 1:1000), rabbit anti-phospho-ATM (S1981) (Abcam, #ab81292, 1:800), rabbit anti-ATM (Abcam, #ab32420, 1:1000), rabbit anti-phospho-53BP1 (S1778) (Cell Signaling Tech, #2675, 1:1000), rabbit anti-53BP1 (Cell Signaling Tech, #4937, 1:1000), mouse anti-TDP-43 (R&D systems, #MAB7778, 1:1000), rabbit anti-phosphoTDP-43 (S409/410) (Proteintech, #22,309–1-AP), mouse anti-Titin (Santa Cruz Biotech, #sc-271946, 1:200), rabbit anti-Stathmin 2 (STMN2; Invitrogen, #720,178, 1:500), mouse anti-GAPDH (Novus, #NBP1-47,339, 1:2000), rat anti-Tubulin (Abcam, #ab6160, 1:2000), and mouse antiβ-actin (Proteintech, #66,009–1-Ig, 1:5000) antibodies.

    Saline:

    Article Title: Endogenous TDP-43 mislocalization in a novel knock-in mouse model reveals DNA repair impairment, inflammation, and neuronal senescence
    Article Snippet: .. After blocking with 5% skimmed milk solution in 1% Tris-Buffered saline with Tween 20 (TBST) buffer, the membranes were immunoblotted with mouse anti-Flag antibody (Sigma, #F3165, 1:1000), rabbit anti-TDP-43 (Proteintech, #10,782–2-AP, 1:1000), mouse anti-phospho-Histone H2AX (S139) (EMD Millipore, #16–193, 1:1000), rabbit anti-H2AX (Cell Signaling Tech, #2595, 1:1000), rabbit anti-phospho-ATM (S1981) (Abcam, #ab81292, 1:800), rabbit anti-ATM (Abcam, #ab32420, 1:1000), rabbit anti-phospho-53BP1 (S1778) (Cell Signaling Tech, #2675, 1:1000), rabbit anti-53BP1 (Cell Signaling Tech, #4937, 1:1000), mouse anti-TDP-43 (R&D systems, #MAB7778, 1:1000), rabbit anti-phospho-TDP-43 (S409/410) (Proteintech, #22,309–1-AP), mouse anti-Titin (Santa Cruz Biotech, #sc-271946, 1:200), rabbit anti-Stathmin 2 (STMN2; Invitrogen, #720,178, 1:500), mouse anti-GAPDH (Novus, #NBP1-47,339, 1:2000), rat anti-Tubulin (Abcam, #ab6160, 1:2000), and mouse anti-β-actin (Proteintech, #66,009–1-Ig, 1:5000) antibodies. .. Protein bands were visualized by probing with corresponding HRP-conjugated or IRDye secondary antibodies and developed with enhanced chemiluminescence reagent or at appropriate IR channels in Odyssey (LI-COR).

    Article Title: Endogenous TDP-43 mislocalization in a novel knock-in mouse model reveals DNA repair impairment, inflammation, and neuronal senescence.
    Article Snippet: .. After blocking with 5% skimmed milk solution in 1% Tris-Buffered saline with Tween 20 (TBST) buffer, the membranes were immunoblotted with mouse anti-Flag antibody (Sigma, #F3165, 1:1000), rabbit anti-TDP-43 (Proteintech, #10,782–2-AP, 1:1000), mouse anti-phospho-Histone H2AX (S139) (EMD Millipore, #16–193, 1:1000), rabbit anti-H2AX (Cell Signaling Tech, #2595, 1:1000), rabbit anti-phospho-ATM (S1981) (Abcam, #ab81292, 1:800), rabbit anti-ATM (Abcam, #ab32420, 1:1000), rabbit anti-phospho-53BP1 (S1778) (Cell Signaling Tech, #2675, 1:1000), rabbit anti-53BP1 (Cell Signaling Tech, #4937, 1:1000), mouse anti-TDP-43 (R&D systems, #MAB7778, 1:1000), rabbit anti-phosphoTDP-43 (S409/410) (Proteintech, #22,309–1-AP), mouse anti-Titin (Santa Cruz Biotech, #sc-271946, 1:200), rabbit anti-Stathmin 2 (STMN2; Invitrogen, #720,178, 1:500), mouse anti-GAPDH (Novus, #NBP1-47,339, 1:2000), rat anti-Tubulin (Abcam, #ab6160, 1:2000), and mouse antiβ-actin (Proteintech, #66,009–1-Ig, 1:5000) antibodies. .. After blocking with 5% skimmed milk solution in 1% Tris-Buffered saline with Tween 20 (TBST) buffer, the membranes were immunoblotted with mouse anti-Flag antibody (Sigma, #F3165, 1:1000), rabbit anti-TDP-43 (Proteintech, #10,782–2-AP, 1:1000), mouse anti-phospho-Histone H2AX (S139) (EMD Millipore, #16–193, 1:1000), rabbit anti-H2AX (Cell Signaling Tech, #2595, 1:1000), rabbit anti-phospho-ATM (S1981) (Abcam, #ab81292, 1:800), rabbit anti-ATM (Abcam, #ab32420, 1:1000), rabbit anti-phospho-53BP1 (S1778) (Cell Signaling Tech, #2675, 1:1000), rabbit anti-53BP1 (Cell Signaling Tech, #4937, 1:1000), mouse anti-TDP-43 (R&D systems, #MAB7778, 1:1000), rabbit anti-phosphoTDP-43 (S409/410) (Proteintech, #22,309–1-AP), mouse anti-Titin (Santa Cruz Biotech, #sc-271946, 1:200), rabbit anti-Stathmin 2 (STMN2; Invitrogen, #720,178, 1:500), mouse anti-GAPDH (Novus, #NBP1-47,339, 1:2000), rat anti-Tubulin (Abcam, #ab6160, 1:2000), and mouse antiβ-actin (Proteintech, #66,009–1-Ig, 1:5000) antibodies.

    Cell-Signaling:

    Article Title: Endogenous TDP-43 mislocalization in a novel knock-in mouse model reveals DNA repair impairment, inflammation, and neuronal senescence
    Article Snippet: .. After blocking with 5% skimmed milk solution in 1% Tris-Buffered saline with Tween 20 (TBST) buffer, the membranes were immunoblotted with mouse anti-Flag antibody (Sigma, #F3165, 1:1000), rabbit anti-TDP-43 (Proteintech, #10,782–2-AP, 1:1000), mouse anti-phospho-Histone H2AX (S139) (EMD Millipore, #16–193, 1:1000), rabbit anti-H2AX (Cell Signaling Tech, #2595, 1:1000), rabbit anti-phospho-ATM (S1981) (Abcam, #ab81292, 1:800), rabbit anti-ATM (Abcam, #ab32420, 1:1000), rabbit anti-phospho-53BP1 (S1778) (Cell Signaling Tech, #2675, 1:1000), rabbit anti-53BP1 (Cell Signaling Tech, #4937, 1:1000), mouse anti-TDP-43 (R&D systems, #MAB7778, 1:1000), rabbit anti-phospho-TDP-43 (S409/410) (Proteintech, #22,309–1-AP), mouse anti-Titin (Santa Cruz Biotech, #sc-271946, 1:200), rabbit anti-Stathmin 2 (STMN2; Invitrogen, #720,178, 1:500), mouse anti-GAPDH (Novus, #NBP1-47,339, 1:2000), rat anti-Tubulin (Abcam, #ab6160, 1:2000), and mouse anti-β-actin (Proteintech, #66,009–1-Ig, 1:5000) antibodies. .. Protein bands were visualized by probing with corresponding HRP-conjugated or IRDye secondary antibodies and developed with enhanced chemiluminescence reagent or at appropriate IR channels in Odyssey (LI-COR).



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